This project focuses on developing a standardized pipeline that integrates microbiological assays with advanced optical microscopy for evaluating bacterial adhesion on novel materials produced by the Research Training Group (RTG) 2723: Materials-Microbes-Microenvironments (M-M-M). The study examines bacterial growth and adhesion on a range of substrates, including titanium (Ti), MoS₂ nanowalls, plastic, and bioactive glass. Genetically modified Staphylococcus aureus strains expressing Green Fluorescent Protein (GFP), Red Fluorescent Protein (RFP), and Far-Red Fluorescent Protein (FRFP) were constructed and cultured on these materials. Bacterial growth was quantified through colony-forming unit (CFU) counting, while fluorescence microscopy provided high-resolution visualization of bacterial colonization. This standardized approach enables robust evaluation of material-bacteria interactions, supporting the development of innovative biomaterials.