TiO₂ nanotube (TNT) tailored surface for enhancing properties of titanium components is an extensively studied topic due to its wide application base among biomedical and energy sectors. The present research consists of a comparative study of TNT growth on four different titanium micro structures ie: pure titanium, rolled Ti6Al4V, heat-treated rolled Ti6Al4V and direct metal laser sintered Ti6Al4V. A variety of components used in biomedical implants, solar cell applications and drug delivery systems use alloys of titanium preferably one of the four sample materials used in this study. A detailed relative study of these samples correlates nanotube growth with actual manufacturing parameters involved in fabricating titanium components and their microstructures. The electrochemical anodization process was used for growing TNT followed by advanced characterization techniques which quantitatively measured nanotube dimensions, constituent elements present, crystal phase and growth orientations. Comparative electrochemical studies for corrosion behavior in simulated body fluid (SBF) solution environment provides significant insight into the microstructural effect on the TNT growth process.