Bioimaging techniques for evaluating cutaneous tumors provide supplementary knowledge to conventional histopathological examination. LIBS (Laser-induced breakdown spectroscopy) is a suitable pre-screening method for large sample sets due to its speed and reproducibility. Conversely, LA-ICP-MS (Laser ablation inductively coupled plasma mass spectrometry) is often considered a core and reference technique for pre-selected samples.

This research highlights the advantages of combining these two techniques, offering complementary data on biotic elements (Ca, Mg, P) and trace elements (Cu, Zn) within tumor tissues. The investigation examined changes in elemental distribution in various tumors, including malignancies (squamous cell carcinoma, basal cell carcinoma, malignant melanoma, epithelioid angiosarcoma), a benign pigmented nevus, and a healthy skin sample.

Several advanced image processing techniques were used to evaluate the measured data, including implementing clustering or numerical correlation.