Ti-6Al-4V alloys are extensively used in biomedical applications such as load bearing hip implants which are subjected to cyclic loading. These implants are often patient-specific to meet the anatomy and fitment requirements which can be readily manufactured using additive manufacturing (AM) techniques. In this work, Ti-6Al-4V ELI specimens were prepared using the direct metal laser sintering (DMLS) technique and their monotonic and cyclic responses were compared with the conventional Ti-6Al-4V ELI material. As built AM specimens were further annealed at 900°C for 2 hrs, followed by furnace cooling to remove residual stresses and fully decompose the martensitic structure. Tensile and stress-controlled fatigue tests were performed for both AM and conventional specimens. The yield strength (897 MPa), ultimate tensile strength (965 MPa), and ductility (11%) of AM specimens were found to be well above the minimum recommended values for surgical implants as per ASTM F136 standard. However, these tensile properties were inferior to conventional material. Similar to the monotonic responses, the fatigue strength of AM specimens (224 MPa) was 35% lower than the conventional specimens. The fractographic images captured using a scanning electron microscope (SEM) for the fractured AM surfaces revealed the presence of several semi-fused particles at crack initiation sites, which suggests the low crack initiation resistance in AM specimens due to the presence of process-induced defects. Overall, the AM specimens qualify the minimum requirements of medical implants, however, compared to conventional specimens, there is further scope for improvement by reducing the process-induced defects.