Stereolithography (SLA)-based 3D printing is a highly useful and still underused tool for fabrication of in vitro 3D tissue mimics. Provided that suitable bioactive and cell compatible 3D printing resins are available, biofabrication with even unsophisticated SLA printers can yield tissue scaffolds with a reasonably high resolution. However, these bioresins have high requirements as they would ideally show an enhanced level of biomimicry and possess a suitable viscosity and photocrosslinking kinetics for the SLA printer. In our work, we formulated a new bioactive photocrosslinkable resin based on methacryl-functionalized decellularized porcine small intestine submucosa (dSIS-MA). The new resin consisting of 1.5 wt-% of dSIS-MA resulted in fully transparent hydrogels with the stiffness value of 3.5 kPa, being thereby close to the stiffness of a human small intestine. With this resin, we first 3D printed acellular dSIS-MA scaffolds that physiologically mimicked a native small intestine and continued then by seeding primary intestinal epithelial cells on the scaffold surface to test its cell compatibility. The seeded epithelial cells isolated from human intestinal crypts grew along the villi mimics forming continuous tight junctions and producing mucin 5AC, demonstrating thereby two main characteristics of a native intestinal epithelium. The unique combination of high printability and elevated biomimicry made the new dSIS-MA resin an excellent material for 3D printing of villi-mimicking intestinal architectures as it enabled the enhanced physiological relevancy and bioactivity of the resulting tissue scaffolds.