Functionally graded gyroid scaffolds based on triply periodic minimal surfaces (TPMS) offer significant potential for osseointegrative implants. These additively manufactured structures, designed with a gradient of porosity and high surface roughness, mimic the complex architecture of human bone. Fabricated from biocompatible Ti6Al4V using electron beam melting (EBM), these scaffolds exhibit enhanced mechanical stability. Their high surface roughness and sinusoidal curvature create an optimal adhesion substrate, allowing cellular attachment with reduced energy expenditure, enhancing ATP availability compared to sharp-edged surfaces. Notably, intracellular oxygen radical levels remain stable, while osteogenic differentiation potential on gyroid surfaces is increased even without added chemical osteogenic stimulation. The addition of osteogenic differentiation promoters further amplifies this positive effect, demonstrating a synergistic benefit with the curved, rough surfaces.