Triboelectric nanogenerators are mechanical to electric energy converter devices based on the phenomenon’s of triboelectrification and charge induction ideal to exploit the wasted energy from the natural movements of the human body. Derived from its fiber/yarn based structure, mechanical properties of textile structures excel most compact sheets or films of equivalent materials in terms of flexibility, elasticity, malleability, moisture permeability and breathability, making it the ideal substrate for wearable electronic devices integration. Single electrode yarn shaped triboelectric energy generator devices (Seys-TENGs) were developed through a new method of depositing PDMS directly onto conductive carbon fiber yarns (in-situ curing method). Such technique allows the fast formation of a uniform thickness-controlled coating over conductive surfaces regardless of their roughness with special interest in the case of fiber shaped electrodes. The incorporation of nanocrystalline cellulose (NCC) films on the structure of Seys-TENGs through a modified electrophoretic deposition process and its ability to enhance the power conversion was studied. Porous and rough layers of NCC, promoted by air bubbles generated during the electrophoretic deposition were obatined. It was found that NCC functionalized Seys-TENGs with NCC-H layers deposited using less than 120 sec did not provide any enhancement and actually degraded the overall power generation. Layers electrodeposited for 120 sec however, resulted in Seys-TENGs with slightly enhanced power conversion capability. The best results were achieved when using a symmetric NCC layer obtained from a deposition time of 120 sec, with a peak-to-peak voltage and current of 34.24 V and 4.45 µA, repectively, while being tested using 30 N of impact force. Further studies using ion doped NCC films where also carried out.