Powder bed-based AM technologies enable a tool-free near-net shape production of complex components directly from a computer-aided design (CAD) file. They are based on the consecutive consolidation of metallic powders and the repetitive fusing of thin layers on top of each other, which provides unprecedented design freedom compared with conventional manufacturing techniques. Besides electron beam-based powder bed fusion (PBF-EB), laser-based powder bed fusion (PBF-LB) represents the most common powder bed based additive manufacturing technique leading to unique microstructures resulting in mechanical properties eventually being significantly different to conventionally processed materials. By using laser sources of different power, the microstructures of components manufactured by PBF-LB can be further tailored to meet possible requirements, e.g. by microstructural gradation. Accordingly, characteristics such as grain size and texture can be influenced by adapting process parameters, and therefore determining the overall mechanical properties. Since the fatigue crack growth (FCG) behavior of a material among others is dependent on the microstructure and process-induced residual stresses, the effects of microstructural gradation on the FCG behavior of AISI 316L specimens manufactured by PBF-LB were investigated in the present study. Graded compact tension (CT) specimens were built with a dual- laser system and differing PBF-LB process parameters, resulting in two distinguishable parts of a specimen, i.e., a coarse-grained and textured area followed by a more fine-grained and less textured area. To reveal the interdependencies between process, microstructure and crack growth properties, FCG tests and microstructural investigations by electron backscattered diffraction (EBSD) were carried out. Evaluation of FCG tests within the Paris and near-threshold regions, with respect to intracrystalline as well as transcrystalline crack growth was conducted. The distinction of aforementioned crack growth types is crucial to understand crack deflection mechanisms. Summed up, the present study aims to convey an understanding of the interplay between process inherent features and microstructural grading with respect to the FCG properties of AISI 316L manufactured by PBF-LB.