The determination of specific proteins is the key to understanding the processes in the organism or the development of diseases. Unfortunately, the determination of heteroatoms as are P, S, Fe, Cu, and Zn is insufficient for this purpose. Hence, the biorecognition tools for their determination by LA-ICP-MS are being developed. We describe labeling the antibodies by gold nanoparticles (AuNPs) with detection by LA-ICP-MS. As the target protein, p53 was chosen due to the high specificity of its appropriate antibody (i.e., DO-1). Nonspecific interactions with the sample matrix – was investigated and optimized using dot blot and western blot assays.