Immuno-mass spectrometry imaging (iMSI) is a technique which enables quantitative, highly multiplexed imaging of proteins in situ. It combines the specificity of antibodies conjugated with isotopically-enriched metals to identify the biomolecule, and the quantitative imaging of LA-ICP-MS. However, the resolution obtainable by LA-ICP-MS is limited by the laser technology, and the low number of metal atoms per antibody, with the signal dropping below the limit of detection for low abundant proteins as cellular resolution is approached. Immunofluorescent (IF) imaging has superior resolution, however is a non-quantitative technique. These techniques are often used for complementary imaging on sequential histological sections, however there may be difficulties overlaying images due to cutting artefacts and non-identical histological regions. Here we developed a multimodal IF and iMSI protocol on the same histological section through sequential application of secondary and tertiary antibodies conjugated with either fluorophores or a metal analyte. Multimodal imaging of the same histological section resulted in improved alignment of the images, and allowed the higher resolution of the IF image to be used to pick pixels, and the underlying iMSI image data the concentration of the protein at that location. Furthermore, signal amplification of both imaging modes could be achieved by applying multiple cycles of the fluorescent secondary and metal-conjugated tertiary antibodies. Amplification is useful for imaging low abundant proteins or for improving resolution, with neurons in murine brain determined via iMSI at sub-cellular 1 µm resolution.