Natural spider silk fibers are one of the toughest known biopolymeric materials combining strength and elasticity. The most investigated spider silk type is the major ampullate (MA) silk, which consists of different proteins known as MA spidroins (MaSps) differing in amino acid composition.  Engineered spidroins inspired by the naturally occurring Araneus diadematus fibroin (ADF) of the European garden spider, could be recombinantly produced by a biotechnological process using E. coli. These proteins are highly processible, for instance in a highly concentrated solution, called dope. Fibers were wet-spun in a microfluidic process upon extrusion of spinning dopes mimicking the natural spinning process.This biomimetic approach yields nature-like performing fibers concerning mechanical features such as tensile strength, elasticity, Young’s modulus as well as toughness.