Chitosan (CS) is well-known as an antibacterial polysaccharide due to its ionic interaction either with the negatively charged bacterial cell or via penetration of chitosan into the nuclei of bacteria leading a reduction of mRNA and protein synthesis. To produce antimicrobial CS coatings, dilute acidic media are required for dissolving CS, however, this complicates further processing. Even though electrophoretic deposition (EPD) is known as a versatile coating method for modifying biomaterial surfaces^1,2, the use of acidic media can significantly increase the conductivity of the suspension, which can negatively affect the deposition rate as the increased number of free ions may act as a primary current carrier or cause water electrolysis, which will reduce the coating quality. In this study, EPD using alternating current (AC) fields is applied to produce antimicrobial CS-based coatings for the first time using water-soluble maleic anhydride modified chitosan (MA-CS) from aqueous media without additional acid. The effectiveness of AC-EPD as a fast deposition tool is compared with the commonly applied diffusion methodology (i.e., classic dipping). AC-EPD CS coatings generate an increased surface topography on the metal substrate for the same processing time compared to dip-coated samples. Thorough surface characterization, combining scanning electron, atomic force microscopy, confocal microscopy, contact angle, infrared spectroscopy, and surface zeta potential analyzer revealed the effectiveness of AC-EPD for generating uniform CS coatings. Moreover, surface physico-chemistry evaluation points out that AC-EPD CS coatings exhibit amphiphilic and isotropic behaviors while other CS-treated samples show an anisotropic monopolar behavior. Last, the biofunctionality of CS-coated metallic substrate produced by AC-EPD in terms of protein adsorption and antimicrobial properties is evaluated. It is shown that AC-EPD CS coatings accelerate a protein adsorption by establishing various interaction with bovine serum albumin and reduce the bacterial biofilm formation against two different strains, one commensal strain (Streptococcus gordonii) and one periopathogenic strain (Porphyromonas gingivalis). Overall, this study shows that AC-EPD allows to concentrate water-soluble MA-CS on titanium without compromising its biological performance, i.e. protein adsorption and antimicrobial properties. This indicates the potential of AC-EPD as a versatile, time-efficient deposition method for polysaccharides in biomedical applications.