The development of cell scaffolds using novel polymeric materials is challenging due to mammalian cells having inconsistent adhesion and proliferation on artificial surfaces, thus 3D printing techniques employ well-known biocompatible materials such as alginates, collagen, and extracellular matrix polymers. This work reports the feasibility of a cell scaffold using a silica-based ink with a fused deposition modeling type 3D-printer and with A431 cells (human epidermoid carcinoma) as a model organism. Fluorescent 3D confocal imaging confirmed that cell proliferation was observed not only on the surface of the material but also in its interior. Furthermore, proteomic analysis of the A431 on the scaffolds showed a distinct profile from that of the traditional flask cultures, indicating that the scaffolded cells would exhibit biophysical responses comparable to organoids or 3D cultures, thus opening the possibility for applications in toxicological and environmental bioassays.