Implant-associated infections are a growing challenge worldwide due to the aging population and the antibiotic resistance of bacteria [1]. Bacterial adhesion and biofilm formation on implant surfaces are considered significant risks for implant-associated infections in clinical applications, resulting in prosthetic joint failure [2]. Ni-free Ti-based bulk metallic glasses (BMGs) are promising biomaterials for dental and orthopedic implant applications thanks to their high corrosion resistance, good biocompatibility, high strength, and low Young’s modulus [3,4]. Moreover, because BMGs have amorphous structures and lack grain boundaries, they can be shaped and patterned by viscous flow deformation using thermoplastic net-shaping (TPN) when heated above glass transition temperature [4,5]. Biomimetic-structured surfaces such as butterfly and cicada wings have been shown to have antifouling properties from anti-adhesive or mechano-bactericidal effects [6]. Likewise, nanopatterned BMGs with different feature sizes have shown influences on cellular responses of various cell types and can be utilized to promote cell-material interactions [7]. Hence, to help host cells win the surface against bacteria, this work aims to create hierarchical structures on the Ti₄₀Zr₁₀Cu₃₄Pd₁₄Sn₂ BMG to enhance osseointegration and antibiofilm properties for dental and orthopedic implants. Polyethylene glycol (PEG) copolymerized coating is applied on the featured Ti-BMG surface to enhance the synergy effect of antibacterial properties for the whole material system. The biocompatibility and antibacterial performance of the patterned BMGs are examined with various in-vitro studies. The biocompatibility with human osteosarcoma Saos-2 cells is investigated via cell viabilities on 1, 3, and 7 days, cell morphology by SEM, and cell adhesion by immunofluorescence detection of tubulin and vinculin. The antibacterial properties are examined to test for effects on the viability of Staphylococcus aureus bacteria with crystal violet assay.

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