Nowadays, laser cladding is a common surface treatment technique applied in various field of industrial applications such as mining- and aviation industry. The applied metal powder that is melted onto to surface is strongly influenced by the chemical composition which has specific properties in terms of weldability. For surfaces that requires high hardness and high ductility, “Ferro 55” coating is chosen for applications that exhibit heavy abrasion, high pressure, and elevated operating temperatures. As a result, the manufactured surface components depict an irregular surface topography with high roughness values, which requires further finishing processes. In addition, different functionalities can be further improved by applying microstructuring methods, for instance using laser-based precision techniques. In this work, direct laser interference patterning (DLIP) is used for manufacturing periodic line-like structures with spatial periods of 6.0 µm on laser cladded Ferro 55 coatings to investigate the influence of different surface roughness on the fabricated microstructures. For the DLIP experiments, an short-pulse laser source with a fundamental wavelength of 1064 nm and a pulse duration of 7 ns is applied. Texture depths up to 4.0 µm were reached, leading to aspect ratios of up to 0.7. The results enable a cost-effective method to tailor the surface architecture, which can lead to an improved component lifetime.