Biofilms can develop on almost all medical implants such as catheters and cardiovascular implants. Due to the biofilm-mediated antimicrobial tolerance, implant-associated infections are difficult to treat. Invertebrate models such as the Galleria mellonella (Greater wax moth) larvae infection model are increasingly being explored to learn more about the pathogenesis of bacterial infections due to less ethical constraints compared to mammalian models. However, implant-associated biofilms have rarely been analysed in the G. mellonella model. The study aimed to evaluate different methods of forming biofilms on medical implants in G. mellonella larvae to mimic implant-associated biofilms.