Real biological tissues show a great variety of different geometric morphologies with special features on different geometric scales. The fabrication of tissue mimicking structures is therefore difficult to realize without the use of multiple time consuming processes. Multiphoton polymerization allows the production of complex 3D structures combining features in the nm-, µm- and cm-range with only one process. Different process parameters like processing speed and laser power as well as the use of different objectives and materials can have tremendous impact on the resolution, stability and biocompatibility of resulting architectures. In this approach, structures that mimic the intricate and highly hierarchical morphology of the microscopic subunit of the liver – the liver lobule - were produced. Objectives with different numerical apertures, different materials and design strategies were explored and the resolution and stability of resulting structures compared. Fluidic simulations showed that the designed hollowed liver lobule mimicking structures have similar fluid behavior compared to that of native tissue. In a next step the fluidic actuation of the designed architectures is planned to achieve scaffolds that can be used as “Cell Sheets” in microfluidic controlled 3D cell cultures.