Single particle ICP-MS has benefitted from utilizing laser ablation to analyze particles directly off solid substrates rather than being limited to liquid suspensions. Laser ablation is a suitable sampling method for these particle transient signals that typically last ~0.5ms when used with millisecond dwell times (for quadrupole-based instruments) or integration times (for time-of-flight instruments). While a handful of studies have looked at factors that may affect signal from LA-spICP-MS, this presentation will discuss the effects of two different lasers; the imageBIO266 (266nm) and the imageGEO193 (193nm). Comparison of these two lasers will also include effects on single particle signal through changing spot size, spot overlap, and raster speed/scan rate. Furthermore, detection of particles of different sizes and elemental compositions in different particles matrices and dissolved backgrounds will be examined. Au nanoparticles and Au-Ag core-shell particles will be used to demonstrate these effects. Iolite software will be used to better map particles and directly compare them to particle numbers obtained through microscopic techniques to determine particle recovery in terms of detected particles using LA-spICP-MS.