In this study, we investigate the interfacial properties on I-modified Au(111) during the Ag UPD in aprotic electrolyte. We observed two sets of Ag UPD peaks in cyclic voltammetry (CV). The charge density for these peaks is about 216 µC/cm² close to the theoretical charge density for the monolayer (222 µC/cm²). We observed the iodine structure at low normal load (F_N < 20nN), which shows that the iodine adlayer on Au(111) and Ag monolayer forms a (˅3×˅3)R30°. It indicates that the iodine adlayer is stable during the Ag UPD. At high normal load, the AFM tip penetrates into iodine layer and reveals the structure of Au(111) or Ag monolayer depending on the potential. It is remarkable that there is no irreversible wear during the penetration of AFM tip meaning that the iodine structure appears again with decreasing normal loads. At high normal load where the tip penetrates into iodine layer and interacts with substrates (Au and Ag), the friction on Ag monolayer is higher than on Au(111). Interestingly, even though the cyclic voltammetry (CV) shows that the amount of water in the electrolyte has minor influence, the friction increases with an increase in water content on the I/Ag/Au(111).