Biofilm formation is a complex phenomenon involving biopolymers and microorganisms. The first step is the formation of a conditioning film by non-specific adsorption of non-microbial components that microbes will later on colonize. Two main components of the conditioning film are proteins and carbohydrates. Here, the adsorption of important salivary proteins (bovine serum albumin (BSA), lysozyme (LYS)) and a polysaccharide (dextran, DEX) on dental titanium have been investigated by a multimethod approach comprising biochemical assays (protein and carbohydrate amounts, lysozyme activity) and surface analytical methods (scanning force microscopy and spec-troscopy, and quartz crystal microbalance). Furthermore, a UV spectrophotometric quantification method was developed to simultaneously determine the total amounts of proteins and polysaccha-rides in the same sample.