The widespread dumping of plastic waste into the environment poses a significant problem for the next several decades, especially with regard to the monitoring and measurement of microplastics (MPs) in diverse environmental matrices and in living organisms. There are several different sources of microplastic contamination, including seafood, food additives, packaging materials, and industrial and agricultural products. Even though MPs have recently been found in a wide range of environmental media and living things, it is still unclear how they disperse and what their toxicological implications are for people. There is increasing evidence that microplastics, which may enter the body through tainted food or packaging materials, can have negative effects on both human health and the environment. Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has great potential in the identification of polymer alterations and marker metals in polymers. Its direct application in MPs detection, however, has been restricted to tracking trace metals adsorbed on MPs. By enhancing MPs ability to be detected and imaged in biological tissues, this research will provide new avenues for monitoring MPs throughout the human body and evaluating any health effects. Also, combining element specific imaging from LA-ICP-MS with µCT volumetry may provide the “golden analytical standard” for exact MPs characterization & distribution in the organ systems of living organisms. In order to reduce possible health hazards, it is critical to comprehend how microplastics interact with biological systems. This presentation highlights imaging two size types of polyethylene terephthalate (PET) MPs, artificially inducted into rat's grounded liver tissue, heart and brain to controllable mimic real MPs accumulation. MPs were directly imaged by analysis Sb & Co as metal markers in the form of MPs additives.