Complex components for high temperature gas turbine applications require material that offer a combination of excellent high-temperature strength and oxidation resistance. Nickel-based superalloys with high gamma prime (g’) volume fractions are particularly suited for these applications, especially combined with additive manufacturing (AM) for complex geometries. Due to the complex thermal history that materials experiences during laser powder bed fusion (LPBF) processing, microstructures differ significantly from traditionally manufactured alloys. This study follows g’ formation in a non-weldable nickel-based superalloy, manufactured by means of LPBF, considering the as-printed material and subsequent post-AM heat treatment. Atom probe tomography (APT) of the as-built material indicated regions of approx. 5 nm which are enriched in Ni, Al, and Ti which show no characteristic superlattice diffraction under transmission electron microscopy (TEM). With an extensive heat treatment matrix, an experimental TTT diagram is created based on scanning electron microscopy (SEM) analysis of the material which is then compared to a calculated diagram. Additional APT and TEM analysis is performed on heat-treated material to elucidate the evolution of the previously observed regions.