This work describe a simple phase separation procedure for structuring of films made from hyaluronan (HU). HU is known for its biocompatibility and finds broad applicability in biomedical, tissue engineering and pharmaceutical research as well as practical use in cosmetics and dermatology.[1] A time-sequenced dispensing of different solution mixtures was applied under rotation on HU covered substrates to generate surface textures without the need for sacrificial removable templates or additives. Texturization of HU films was achieved through the application of two methods: phase separation and phase inversion. In the phase separation process, a mixture of good and poor solvents was applied to the surface,[2,3] resulting in the formation of surface relief on the scale of micrometers to submicrometers. Conversely, in the phase inversion approach, a solvent mixture, along with HU, was applied to a rotating surface, generating surface topography with features on the scale of submillimeters. Both methods are closely dependent on both the process parameters of the procedure and the molecular weight of the HU used. Thin HU films modified by phase separation exhibit improved mechanical properties. Together with the created surface relief featuring a higher specific surface area, this could represent an effective carrier for drug delivery.