Near-net shape manufacturing using powder metallurgy (PM) and hot isostatic pressing (HIP) technique provides the advantages of producing components to precise tolerances and eliminating production time, machining cost, and wasted materials. This production route is applicable in different industries such as aerospace, automotive, and marine to produce components with different sizes and shapes. At the last step of the HIP cycle, the capsule -which set the shape of the component- is removed using an acid-leaching bath. This changes the outermost layers of the final product. Generally, manufacturers prefer to reduce the post-machining procedure as much as possible. A few researchers tried to optimize this post-machining process. However, it is still not clear how the surface quality (pickling without machining) influences the fatigue properties. In this study, the effect of the surface quality of HIPed Inconel 625 samples on high cycle fatigue behavior was investigated. Two different surface conditions of i) machined surface and ii) pickled surface were studied. The surface roughness measurements were carried out on 5 samples of each condition. Four-point bending test was performed under a stress ratio of 0.1 and a frequency of 10 Hz in order to achieve fatigue strength at 10⁶ cycles. The fatigue strengths were obtained using the staircase method. The results revealed the fatigue strengths of 260 ± 8.2 and 236 ± 45 MPa for machined and pickled surfaces, respectively. Based on these results, the higher surface roughness and lower surface quality affected the standard deviation much more than the mean fatigue strength, such that the pickled surface showed a 10% lower fatigue strength, but with a more than 5 times higher standard deviation compared to the machined one. Fractography of the samples confirmed that 90% of the pickled samples failed due to surface pickling defects; however, the reason of failure for more than 90% of the machined samples was inclusions. Failure defect size distributions were collected and related to their harmfulness in the two different conditions. It could be concluded that performing a machining procedure after the HIP process is vital to achieving more confident and predictable fatigue properties.