Over the years, overuse and misuse of antibiotics has lead to emergence of multidrung resistance (MDR) in microorganims. The rapid increase of MDR in the recent years is the root cause for a growing number of nosocomial infections. The treatment of these infections has, however, become a challenge due to ineffectiveness of antibiotics. In such cases, utilization of antibacterial materials in combination with novel surface modifcation techniques can be extremely beneficial to hinder the spread of infections. Copper is known to exhibit very beneficial properties as regard to contact killing of bacteria. However, the application for pure copper has its limitation such as mechanical strength and corrosion. This can be modified by alloying, as it is a powerful tool in altering the properties of a material according to the required application. In course of this research, surfaces of various copper-based alloys were modified with ultrashort pulsed direct laser interference patterning (USP-DLIP) technique to produce periodic line structures. The periodicity was chosen in accordance to dimension of the tested bacteria (see Fig.1). At first, the influence of alloying on laser material interaction was investigated and analyzed via several charaterization methods. After that, the surface was tested via Contact Killing method to determine the effect of surface modification on the antibacterial activity. Overall, a series of experiments were performed to determine the correlation between the structures produced via USP-DLIP and the antibacterial properties. The obtained results will also be beneficial for understanding the effect of alloying on the antibacterial efficiency.