Mycelium materials are versatile materials offering a wide range of properties depending on parameters including substrate, growth conditions or strain used. More specifically, in the case of leather like biomaterials made of pure mycelium, finding an optimal strain is critical. Pure mycelium materials are made of one and only component: hyphae. The hyphal system of fungi can be divided into three different types: monomitic, dimitic and trimitic, based on the capability of each strain to produce generative, generative and skeletal, or generative, skeletal and ligative hyphae. It has been proven that skeletal hyphae in fungal fruiting bodies provide the most significant contribution to the mechanical strength (69% of the compression modulus) and that ligative hyphae was second in this contribution (19%). In this study, we observed the hyphal structure of mycelium materials produced through Liquid State Surface Fermentation and decyphered the cell organization of these materials. Strains from a strain pool featuring monomitic, dimitic or trimitic hyphal systems were used to produce Pure Mycelium Mats and observed under the microscope (optic and SEM) and tested for tensile strength and hydrophobicity (Water Contact Angle). By using a pure mycelium materials produced by the same strain and composed of generative versus skeletal hyphae, we could assess the contribution of each type of cells in a mat. By linking mycology to mycelium materials science, this preliminary study provides insights on how to assess if a fungal strain could potentially have desirable mechanical properties for the growth of leather like materials.