Bioprinting introduces an approach to fabricate targeted cell-embedded tissues developed by biocompatible polymers, promoting highly controlled deposition of cells and biomolecules inside the hydrogel. The liability of designed functional models is entangled in gaining the highest precision leading to higher cell viability inside the mimicked microstructures and achieving a multi-structural cell incorporation more similar to a natural organ. Our main goal is to create highly precise patterned cell-laden hydrogels using high cell density by droplet-based bioprinting (DBB), to achieve a functional cardiac tissue model, providing a mimicked environment for cells to advance cardiac tissue engineering in the context of the cardiac lesion.The methodology for the investigation of cardiac models included the characterization of printing parameters such as pressure and valve opening time (VOT) during bio ink dispensing by using different bioprinters performing droplet-based bioprinting. The basis of the patterned models were alginate and fibrinogen-based hydrogels at various concentrations which after combining with immortalized human ventricular fibroblast (HVF) and mesenchymal stem cells (BM-hMSC) cells, were printed. The engineered tissue models as well as the viability of cells inside the bioprinted microstructures were investigated. The results showed the highest precision achieved by optimization of the valve opening time (VOT) and dosing distance while dispensing alginate and Fibrinogen-based hydrogels. By monitoring the applied pressure, bioink rheology, and cell density, we succeeded in patterning highly functionalized cardiac tissue models with high resolution which steps forward into co-culture studies for functional tissue models development.