Exploring a cutting-edge field of materials science and biomedical engineering, nano-structured surfaces emerge as a forefront domain with significant implications. Antimicrobial materials play a vital role in addressing microbial infections associated with biomaterial interfaces, which are commonly found in medical implants, devices, and other healthcare applications. These infections are a major clinical challenge, as they are difficult to treat and often require implant replacement. Current antimicrobial strategies are mainly based on the use of antibiotic drugs. Antibiotic-free antimicrobial biomaterials offer a promising solution for preventing BAIs without contributing to antibiotic resistance. Materials with physical antimicrobial actions are especially effective, providing early infection prevention. Preliminary results indicate that nano-adhesion points on biomaterial surfaces can reduce microbial adhesion forces. Using hydrothermal treatment, we created nanostructures that mechanically damage microbial cell membranes and help to reduce the microbial adhesion in early adhesion stages. To evaluate this, we comprehensively tested the effects of various nano topologies on microbial adhesion. Additionally, the proliferation of osteoblasts was analysed and compared on varying topologies at different time points.